Controlling ligand surface density on streptavidin-magnetic particles by a simple, rapid, and reliable chemiluminescent test

Abstract

the Agence National de la Recherche (ANR-15-CE17-0005-01) and the Spanish Ministry of Education (CTQ2016-79412-P). D.B.-R. acknowledges the Ph.D. grant (BP14-137) from Asturias Regional Government (Spain) and the mobility grant convened by the University of Oviedo and financed by Banco Santander. Paris Descartes University, INSERM, and CNRS are also acknowledged. We would also thank to Dr. J. F. Gaucher, Dr. W-Q. Liu, and Dr. R. Gahoual for kindly providing the biotinylated VEGF95, peptides, and TNFα protein, respectively. Finally, the UMR 8258-CNRS is acknowledged for sharing the TECAN SAFIRE plate-reader.