Open Access BASE2019

Nuclear and chloroplastic SNP markers for genetic studies of timber origin for Hymenaea trees

Chaves, Camila L; Blanc-Jolivet, Celine; Sebbenn, Alexandre M; Mader, Malte; Meyer-Sand, Barbara R. V; Paredes-Villanueva, Kathelyn; Honorio Coronado, Euridice N; Garcia-Davila, Carmen; Tysklind, Niklas; Troispoux, Valerie; Massot, Marie; Degen, Bernd

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Abstract

German Federal Ministry of Food and Agriculture (BMEL) ; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) ; Investissements d'Avenir, Convention attributive d'aide EquipEx Xyloforest ; European Union, FEDER ; Investissements d'Avenir, Convention attributive d'aide EquipEx Xyloforest: ANR-10-EQPX-16-01 ; European Union, FEDER: ANR-10-EQPX-16-01 ; European Union, FEDER: 2003227 ; European Union, FEDER: ANR-10-LABX-25-01 ; We developed nuclear and chloroplastic single nucleotide polymorphism (SNP) and INDEL (insertion/deletion) markers using restriction associated DNA sequencing (RADSeq) and low coverage MiSeq genome sequencing to set up a genetic tracking method of the geographical origin of Hymenaea sp. From two initial sets of 358 and 32 loci used to genotype at least 94 individuals, a final set of 75 nSNPs, 50 cpSNPs and 6 INDELs identifying significant population structure was developed.

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