Dietary supplementation of heat-treated Gracilaria and Ulva seaweeds enhanced acute hypoxia tolerance in gilthead sea bream (Sparus aurata)

Open Access

Abstract

Intensive aquaculture practices involve rearing fish at high densities. In these conditions, fish may be exposed to suboptimal dissolved O levels with an increased formation of reactive O species (ROS) in tissues. Seaweeds (SW) contain biologically active substances with efficient antioxidant capacities. This study evaluated the effects of dietary supplementation of heat-treated SW (5% Gracilaria vermiculophylla or 5% Ulva lactuca) on stress bioindicators in sea bream subjected to a hypoxic challenge. 168 fish (104.5 g average weight) were distributed in 24 tanks, in which eight tanks were fed one of three experimental diets for 34 days: (i) a control diet without SW supplementation, (ii) a control diet supplemented with Ulva, or (iii) a control diet with Gracilaria. Thereafter, fish from 12 tanks (n=4 tanks/dietary treatment) were subjected to 24 h hypoxia (1.3 mg O l) and subsequent recovery normoxia (8.6 mg O l). Hypoxic fish showed an increase in hematocrit values regardless of dietary treatment. Dietary modulation of the O-carrying capacity was conspicuous during recovery, as fish fed SW supplemented diets displayed significantly higher haemoglobin concentration than fish fed the control diet. After the challenge, survival rates in both groups of fish fed SW were higher, which was consistent with a decrease in hepatic lipid peroxidation in these groups. Furthermore, the hepatic antioxidant enzyme activities were modulated differently by changes in environmental O condition, particularly in sea bream fed the Gracilaria diet. After being subjected to hypoxia, the gene expression of antioxidant enzymes and molecular chaperones in liver and heart were down regulated in sea bream fed SW diets. This study suggests that the antioxidant properties of heat-treated SW may have a protective role against oxidative stress. The nature of these compounds and possible mechanisms implied are currently being investigated. ; This work has been carried out with financial support from the European Commission of the European Union, specific RTD Seventh Framework Programme under grant projects SEABIOPLAS (606032), AQUAEXCEL (262336) under TNA project 0132/09/04/14b (ALGAE-SPARUS). L.J.M. is supported by a Fundação para a Ciência e a Tecnologia (FCT) research position with exploratory grant IF/01314/2014/CP1231/CT0001 from Portugal. J.A.M.-S. is supported by a Postdoctoral Research Fellowship (Juan de la Cierva-Formación, reference FJCI-2014-20161) from the Spanish Ministry of Economy and Competitiveness. ; Peer reviewed