Boron Tolerance in Aspergillus nidulans Is Sustained by the SltA Pathway Through the SLC-Family Transporters SbtA and SbtB

Abstract

Microbial cells interact with the environment by adapting to external changes. Signal transduction pathways participate in both sensing and responding in the form of modification of gene expression patterns, enabling cell survival. The filamentous fungal-specific SltA pathway regulates tolerance to alkalinity, elevated cation concentrations and, as shown in this work, also stress conditions induced by borates. Growth of sltA − mutants is inhibited by increasing millimolar concentrations of boric acid or borax (sodium tetraborate). In an attempt to identify genes required for boron-stress response, we determined the boric acid or borax-dependent expression of sbtA and sbtB , orthologs of Saccharomyces cerevisiae bor 1, and a reduction in their transcript levels in a Δ sltA mutant. Deletion of sbtA , but mainly that of sbtB , decreased the tolerance to boric acid or borax. In contrast, null mutants of genes coding for additional transporters of the Solute Carrier (SLC) family, sB , sbtD or sbtE , showed an unaltered growth pattern under the same stress conditions. Taken together, our results suggest that the SltA pathway induces, through SbtA and SbtB, the export of toxic concentrations of borates, which have largely recognized antimicrobial properties. ; Work at CIB-CSIC was funded by MINECO project BFU2012-33142 and MINECO/FEDER/EU project BFU2015-66806-R to E.A.E. Work at the UPV/EHU lab was funded by the Basque Government (grant IT599-13 to U.U.) and UPV/EHU (grant EHUA15/08 to O.E.). M.V. held a contract under project BFU2012-33142. ; We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI).