TY  - JOUR
TI  - Environmental monitoring of SARS-CoV-2 in the metropolitan area of Porto Alegre, Rio Grande do Sul (RS), Brazil
AU  - Dutra, Leticia Batista
AU  - Stein, Janaína Francieli
AU  - da Rocha, Bruna Seixas
AU  - Berger, Andresa
AU  - de Souza, Beatriz Andrade
AU  - Prandi, Bruno Aschidamini
AU  - Mangini, Arthur Tonietto
AU  - Jarenkow, André
AU  - Campos, Aline Alves Scarpellini
AU  - Fan, Fernando Mainardi
AU  - de Almeida Silva, Maria Cristina
AU  - Lipp-Nissinen, Katia Helena
AU  - Loncan, Manuel Rodrigues
AU  - Augusto, Matheus Ribeiro
AU  - Franco, Ana Cláudia
AU  - de Freitas Bueno, Rodrigo
AU  - Rigotto, Caroline
PY  - 2023
PB  - Springer Science and Business Media LLC
LA  - eng
AB  - AbstractSince starts the coronavirus disease 2019 (COVID-19) pandemic identified the presence of genomic fragments of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in various environmental matrices: domestic sewage, surface waters, and contaminated freshwater. Environmental monitoring of SARS-CoV-2 is a tool for evaluating trend curves over the months, compared to several clinical cases of the disease. The objective of this study was to monitor the SARS-CoV-2 in environmental samples collected in different sites in a metropolitan area of Porto Alegre, Southern Brazil. During 10 months from 2020 to 2021, 300 samples were collected weekly and biweekly from nine points located in 3 cities: one point from a wastewater treatment plant (WWTP) in São Leopoldo (fortnightly collection), two points in Dilúvio Stream in Porto Alegre (fortnightly collection), two points in Pampa and Luiz Rau Streams (weekly collection), and two points in public fountains (fortnightly collection) in Novo Hamburgo. After collection, samples were concentrated by ultracentrifugation, and viral nucleic acids were extracted using MagMax® Core Nucleic Acid Purifications kits and submitted to RT-qPCR, using E, N1, and N2 gene targets of SARS-CoV-2. Only 7% (3/41) samples from public fountains were positive, with a mean viral load (VL) of SARS-CoV-2 RNA of 5.02 × 101 gc/l (2.41~8.59 × 101 gc/l), while the streams had average VL of 7.43 × 105 gc/l (Pampa), 7.06 × 105 gc/l (Luiz Rau), 2.01 × 105 gc/l (Dilúvio), and 4.46 × 105 cg/l (WWTP). The results showed varying levels of viral presence in different sample types, with a demonstrated correlation between environmental viral load and clinical COVID-19 cases. These findings contribute to understanding virus persistence and transmission pathways in the environment. Continuous monitoring, especially in less developed regions, is crucial for early detection of vaccine resistance, new variants, and potential COVID-19 resurgence.
UR  - https://doi.org/10.1007/s11356-023-31081-8
DO  - 10.1007/s11356-023-31081-8
T2  - Environmental science and pollution research: ESPR
VL  - 31
IS  - 2
SN  - 1614-7499
SN  - 0944-1344
SP  - 2129-2144
UR  - https://www.pollux-fid.de/r/cr-10.1007/s11356-023-31081-8
H1  - Pollux (Fachinformationsdienst Politikwissenschaft)
ER  -