Transcriptomic analysis of calcium remodeling in colorectal cancer
Abstract
This article belongs to the Special Issue Latest Advances in Colorectal Cancer Research: Molecular Mechanisms and Therapies. ; Colorectal cancer (CRC) cells undergo the remodeling of intracellular Ca homeostasis, which contributes to cancer hallmarks such as enhanced proliferation, invasion and survival. Ca remodeling includes critical changes in store-operated Ca entry (SOCE) and Ca store content. Some changes have been investigated at the molecular level. However, since nearly 100 genes are involved in intracellular Ca transport, a comprehensive view of Ca remodeling in CRC is lacking. We have used Next Generation Sequencing (NGS) to investigate differences in expression of 77 selected gene transcripts involved in intracellular Ca transport in CRC. To this end, mRNA from normal human colonic NCM460 cells and human colon cancer HT29 cells was isolated and used as a template for transcriptomic sequencing and expression analysis using Ion Torrent technology. After data transformation and filtering, exploratory analysis revealed that both cell types were well segregated. In addition, differential gene expression using R and bioconductor packages show significant differences in expression of selected voltage-operated Ca channels and store-operated Ca entry players, transient receptor potential (TRP) channels, Ca release channels, Ca pumps, Na/Ca exchanger isoforms and genes involved in mitochondrial Ca transport. These data provide the first comprehensive transcriptomic analysis of Ca remodeling in CRC. ; This work was supported by grants BFU2015-70131-R from Ministry of Economy and Competitivity of Spain to Carlos Villalobos and VA145U13. from Regional Government of Castilla y León, Spain to Lucía Núñez. Enrique Pérez-Riesgo is supported by a fellowship from the Spanish Association Against Cancer (AECC). Daniel Ubierna is supported by predoctoral fellowships from Junta de Castilla y León, Spain and the EU Social Fund. Lucía G. Gutiérrez is supported by a predoctoral fellowship from the University of Valladolid, Spain ; We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI). ; Peer Reviewed
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