Suchergebnisse

AbstractWhat can an "ethnographic sensibility" contribute to research on climate change governance? With its emphasis on meaning making and understanding what may lie beneath more obvious interactions and processes, ethnographic methodologies, particularly collaborative event ethnography, are increasingly deployed to address complex questions and achieve conceptual leverage on issues related to climate governance. Drawing on literature in climate anthropology, material geography, and political ethnography, and with examples from our own fieldwork experiences, we devise a heuristic typology underpinned by an ethnographic sensibility to help guide the fieldwork phase of a research project. Building on the well-established practice of hanging out, we introduce hanging around, which attends to spatiality and matter; hanging in, which addresses issues of access and trust; and hanging back to guide the practice of reflexivity. We articulate what fieldwork with an ethnographic sensibility entails and discuss its potential and implications for climate governance research.

Proactive identification and characterization of hazards attributable to chemicals are central aspects of risk assessments. Current legislations and trends in predictive toxicology advocate a transition from in vivo methods to nonanimal alternatives. For skin sensitization assessment, several OECD validated alternatives exist for hazard identification, but nonanimal methods capable of accurately characterizing the risks associated with sensitizing potency are still lacking. The GARD (Genomic Allergen Rapid Detection) platform utilizes exposure-induced gene expression profiles of a dendritic-like cell line in combination with machine learning to provide hazard classifications for different immunotoxicity endpoints. Recently, a novel genomic biomarker signature displaying promising potency-associated discrimination between weak and strong skin sensitizers was proposed. Here, we present the adaptation of the defined biomarker signature on a gene expression analysis platform suited for routine acquisition, confirm the validity of the proposed biomarkers, and define the GARDpotency assay for prediction of skin sensitizer potency. The performance of GARDpotency was validated in a blinded ring trial, in accordance with OECD guidance documents. The cumulative accuracy was estimated to 88.0% across 3 laboratories and 9 independent experiments. The within-laboratory reproducibility measures ranged between 62.5% and 88.9%, and the between-laboratory reproducibility was estimated to 61.1%. Currently, no direct or systematic cause for the observed inconsistencies between the laboratories has been identified. Further investigations into the sources of introduced variability will potentially allow for increased reproducibility. In conclusion, the in vitro GARDpotency assay constitutes a step forward for development of nonanimal alternatives for hazard characterization of skin sensitizers.